immunohistochemistry ihc staining 764 images  (Human Protein Atlas)

Journal: Aging Cell

Article Title: Multi‐Omics Reveals Dysregulated Neurotransmitter Systems in Aging and CNS Disorders

doi: 10.1111/acel.70544

Figure Lengend Snippet: Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) Immunohistochemical staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.

Article Snippet: Immunohistochemical staining images and frontal cortex RNA‐seq data from the Human Protein Atlas (HPA) provided supportive cross‐dataset validation for the snRNA‐seq findings.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Clinical and Experimental Medicine

Article Title: TMEM132A is associated with metabolic reprogramming, macrophage-oriented immune remodeling, and breast cancer progression

doi: 10.1007/s10238-026-02125-3

Figure Lengend Snippet: TMEM132A is upregulated in breast cancer and correlates with clinicopathological features ( A ) Differential expression of TMEM132A between breast cancer tissues and normal breast tissues in the TCGA_BRCA cohort. ( B ) TMEM132A expression comparison between tumor samples from TCGA_BRCA and normal breast tissues from the integrated TCGA and GTEx cohort. ( C-E ) Validation of elevated TMEM132A expression in breast cancer using independent GEO datasets, including GSE20711 ( C ), GSE22820 ( D ), and GSE162228 ( E ). ( F , G ) ROC curve analyses evaluating the diagnostic performance of TMEM132A in distinguishing breast cancer tissues from normal tissues in the TCGA_BRCA cohort ( F ) and the TCGA+GTEx_BRCA cohort ( G ). ( H ) Paired analysis of TMEM132A expression between matched tumor and adjacent normal breast tissues in the TCGA_BRCA cohort. ( I , J ) Association between TMEM132A expression and clinical stage in the TCGA_BRCA cohort. ( K ) Distribution of TMEM132A expression across different molecular subtypes of breast cancer in the TCGA_BRCA cohort. ( L-T ) Relationship between TMEM132A expression and histological grade in the TCGA_BRCA cohort (L) and multiple independent GEO datasets, including GSE7390 ( M ), GSE11121 ( N ), GSE20711 ( O ), GSE21653 ( P ), GSE22219 ( Q ), GSE25055 ( R ), GSE45255 ( S ), GSE42568 ( T ), and GSE61304 ( U ). ( U , V ) Comparison of TMEM132A expression between non-metastatic (M0) and metastatic (M1) breast cancer samples in the TCGA_BRCA cohort ( U ) and the GSE20685 dataset ( V ). ( W ) Distribution of TMEM132A expression levels across breast cancer molecular. subgroups in the TCGA_BRCA cohort. ( X ) Representative immunohistochemical staining images showing TMEM132A protein expression in normal breast tissue and breast cancer tissue obtained from the Human Protein Atlas. ( Y ) Immunofluorescence staining showing TMEM132A expression (green), nuclei (blue), microtubules (red), and merged images in the MCF7 breast cancer cell line

Article Snippet: Fig. 2 TMEM132A is upregulated in breast cancer and correlates with clinicopathological features ( A ) Differential expression of TMEM132A between breast cancer tissues and normal breast tissues in the TCGA_BRCA cohort. ( B ) TMEM132A expression comparison between tumor samples from TCGA_BRCA and normal breast tissues from the integrated TCGA and GTEx cohort. ( C-E ) Validation of elevated TMEM132A expression in breast cancer using independent GEO datasets, including GSE20711 ( C ), GSE22820 ( D ), and GSE162228 ( E ). ( F , G ) ROC curve analyses evaluating the diagnostic performance of TMEM132A in distinguishing breast cancer tissues from normal tissues in the TCGA_BRCA cohort ( F ) and the TCGA+GTEx_BRCA cohort ( G ). ( H ) Paired analysis of TMEM132A expression between matched tumor and adjacent normal breast tissues in the TCGA_BRCA cohort. ( I , J ) Association between TMEM132A expression and clinical stage in the TCGA_BRCA cohort. ( K ) Distribution of TMEM132A expression across different molecular subtypes of breast cancer in the TCGA_BRCA cohort. ( L-T ) Relationship between TMEM132A expression and histological grade in the TCGA_BRCA cohort (L) and multiple independent GEO datasets, including GSE7390 ( M ), GSE11121 ( N ), GSE20711 ( O ), GSE21653 ( P ), GSE22219 ( Q ), GSE25055 ( R ), GSE45255 ( S ), GSE42568 ( T ), and GSE61304 ( U ). ( U , V ) Comparison of TMEM132A expression between non-metastatic (M0) and metastatic (M1) breast cancer samples in the TCGA_BRCA cohort ( U ) and the GSE20685 dataset ( V ). ( W ) Distribution of TMEM132A expression levels across breast cancer molecular. subgroups in the TCGA_BRCA cohort. ( X ) Representative immunohistochemical staining images showing TMEM132A protein expression in normal breast tissue and breast cancer tissue obtained from the Human Protein Atlas. ( Y ) Immunofluorescence staining showing TMEM132A expression (green), nuclei (blue), microtubules (red), and merged images in the MCF7 breast cancer cell line We next assessed the potential diagnostic value of TMEM132A in distinguishing tumor from normal tissues.

Techniques: Quantitative Proteomics, Expressing, Comparison, Biomarker Discovery, Diagnostic Assay, Immunohistochemical staining, Staining, Immunofluorescence